Cloning-Growth and Plasmid Extraction by Alkaline Lysis Method

 

Learning Objectives

• To apply the sterile technique while growing the bacterial culture.

• To perform properly the steps of how to isolate plasmid DNA from bacteria using the plasmid extraction alkaline lysis method for plasmid isolation protocol.
• To understand the role of the devices, the reagents, and the enzymes used in the plasmid extraction by alkaline lysis method.

• To implement proper storage of samples to understand the purpose of plasmid isolation.